Journal: International Journal of Molecular Sciences

Article Title: Polyoxometalate K 6 [P 2 Mo 18 O 62 ] Inactivates Escherichia coli O157:H7 by Inducing recA Expression and Apoptosis-like Bacterial Death

doi: 10.3390/ijms241411469

Figure Lengend Snippet: Polyoxometalate K 6 [P 2 Mo 18 O 62 ] treatment induced characteristic apoptosis-like bacterial death events. ( A ) Quantitation of TUNEL staining by flow cytometry. ( B ) Quantitation of annexin V-FITC/propyl iodide staining by flow cytometry.

Article Snippet: The bacteria were then washed twice with PBS and labelled using a One Step TUNEL Apoptosis Assay Kit (containing a mixture of terminal deoxynucleotidyl transferase and FITC-conjugated dUTP) (Beyotime, Shanghai, China).

Techniques: Quantitation Assay, TUNEL Assay, Staining, Flow Cytometry

Journal: Frontiers in Cell and Developmental Biology

Article Title: BRG1 Links TLR4 Trans-Activation to LPS-Induced SREBP1a Expression and Liver Injury

doi: 10.3389/fcell.2021.617073

Figure Lengend Snippet: BRG1 deficiency in hepatocyte alleviates LPS-induced liver injury. WT and BRG1 LKO mice were injected peritoneally with LPS (15 mg/kg) and sacrificed 24 h after the injection. (A) Plasma ALT levels. N = 5 mice for the saline groups and N = 8 mice for the LPS groups. (B) Plasma AST levels. N = 5 mice for the saline groups and N = 8 mice for the LPS groups. (C) Paraffin sections were stained with F4/80. N = 6 mice for each group. (D) Paraffin sections were stained with TUNEL. N = 6 mice for each group. (E) Gene expression levels were examined by qPCR. N = 6 mice for each group.

Article Snippet: Briefly, paraffin sections were incubated in the TUNEL reaction mixture (R&D Systems, 4810-30-K) at 37°C for 60 min. After several rinses with PBS, the sections were incubated with an anti-HRP antibody at room temperature for 30min.

Techniques: Injection, Clinical Proteomics, Saline, Staining, TUNEL Assay, Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: BRG1 Links TLR4 Trans-Activation to LPS-Induced SREBP1a Expression and Liver Injury

doi: 10.3389/fcell.2021.617073

Figure Lengend Snippet: TLR4 over-expression or SREBP1a over-expression restores liver injury in BRG1 deficient mice. (A–F) WT and BRG1 LKO mice were injected via tail veinadenovirus carrying either TLR4 (Ad-GFP-TLR4), SREBP1a (Ad-GFP-SREBP1a), or GFP (Ad-GFP). 2 week later, the mice were injected with injected peritoneally with LPS (15 mg/kg) and sacrificed 24 h after the injection. Gene expression levels were examined by Western (A) . Plasma ALT levels (B) . Plasma AST levels (C) . Paraffin sections were stained with F4/80 (D) . Paraffin sections were stained with TUNEL (E) . Gene expression levels were examined by qPCR (F) . N = 6 mice for all groups. (G) Primary hepatocytes were isolated from WT or BRG1 LKO mice and transduced with indicated andenoviral particles. The cells were with LPS (1 μg/ml) for 12 h and gene expression was examined by qPCR.

Article Snippet: Briefly, paraffin sections were incubated in the TUNEL reaction mixture (R&D Systems, 4810-30-K) at 37°C for 60 min. After several rinses with PBS, the sections were incubated with an anti-HRP antibody at room temperature for 30min.

Techniques: Over Expression, Injection, Gene Expression, Western Blot, Clinical Proteomics, Staining, TUNEL Assay, Isolation, Transduction

Journal: Drug Design, Development and Therapy

Article Title: Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Improves Spinal Cord Function After Injury in Rats by Activating Autophagy

doi: 10.2147/DDDT.S237502

Figure Lengend Snippet: BMSC-Exos promote functional behavior recovery after SCI and reduce neuronal apoptosis. ( A ) BBB scores at different time points after SCI (n=8/group). ( B ) Representative footprint of animal walking at 28 days after SCI (n=5/group). Blue: paw print of the front paw; red: paw print of the hind paw. ( C, D ) TUNEL staining showed that TUNEL-positive neurons in the BMSC-Exos group were markedly reduced compared to that of the SCI group. A typical photomicrograph of a TUNEL-positive neuron is shown (Injured area). ( E, F ) The expression of apoptosis-related protein after SCI. Relative to the SCI group, the level of cleaved caspase-3 was significantly decreased and that of Bcl-2 was increased in the BMSC-Exos group. *p<0.05, relative to the Sham group; # p<0.05, relative to the SCI group.

Article Snippet: After the sections were fixed, ruptured, and blocked, they were incubated at 37 °C for 1 h with the colorimetric TUNEL reaction mixture (Promega), stained with DAPI, and analyses conducted under a fluorescence microscope.

Techniques: Functional Assay, TUNEL Assay, Staining, Expressing